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My CartReactivity：Rat,Mouse,Human
Application：ELISA,FCM,IP,IF,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：29126
Host：Rabbit
Qty：
Size：
| Name | Anti-PD-L1/CD274 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:300-1200.IF 1:10-100.IP 0.5-4.0 ug for IP and 1:500-2000 for WB |
| Target | PD-L1/CD274 |
| Background | Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, it is predicted to be 27-30 kDa after signal peptide cleavage (PMID: 25609200; 17076679). The apparent molecular weight has also been reported as 45-70 kDa, major glycosylated form of 45-50 kDa and multiple post-translational modifications form of 65-70 kDa (PMID: 18760278; 16493058). |
| Swiss Prot | Q9NZQ7 |
| Gene ID | 29126 |
| Subcellular Location | Cell membrane |
| Immunogen | Recombinant protein of human PD-L1/CD274 |
| Public Immunogen Range | 25-241aa |
| Calculated MW | 33kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IP IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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