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My CartReactivity：Human
Application：IF,WB,IHC,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：157285
Host：Rabbit
Qty：
Size：
| Name | Anti-PRAG1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IF 1:50-200. IHC 1:50-200. ELISA 1:3000-30000. |
| Target | PRAG1 |
| Background | Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (By similarity). |
| Swiss Prot | Q86YV5 |
| Gene ID | 157285 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of Human PRAG1 |
| Public Immunogen Range | 240-290/1406aa |
| Calculated MW | 150kDa |
| Observed MW | 100kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. ELISA 1:3000-30000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB IHC ELISA IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PRAG1 antibody diluted at 1:2000;Lane: U-251MG,MG63.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with PRAG1 antibody diluted at 1:100.
Immunofluorescence analysis of A431 cells using PRAG1 antibody diluted at 1:50.
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