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My CartReactivity：Mouse,Human
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：1656
Host：Rabbit
Qty：
Size：
| Name | Anti-DDX6 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chicken; Orangutan |
| Dilution Ratio | WB 1:1000-3000.; IHC 1:100-300. |
| Target | DDX6 |
| Background | Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts. |
| Swiss Prot | P26196 |
| Gene ID | 1656 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human DDX6 |
| Public Immunogen Range | 1-350aa |
| Calculated MW | 54kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Mouse Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with DDX6 antibody diluted at 1:2000;Lane: HEK293,K-562,Mouse testis.
Immunohistochemistry of paraffin-embedded Human testis cancer with DDX6 antibody diluted at 1:200.
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