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My CartReactivity：Mouse
Application：ELISA,FCM,IF,ICC,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：166647
Host：Rabbit
Qty：
Size：
| Name | Anti-GPR125 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Human;Rat;Dog;Cow;Horse;Sheep |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. ICC 1:100-500. IF 1:100-500. |
| Target | GPR125 |
| Background | G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR125 (G protein-coupled receptor 125), also known as PGR21 or TEM5L, is a 1,321 amino acid multi-pass membrane protein belonging to the G-protein coupled receptor 2 family and the LN-TM7 subfamily. Considered a novel orphan adhesion-type G-protein-coupled receptor, GPR125 has five leucine rich repeats (LRR), an immunoglobulin (Ig) domain and a GPS domain. GPR125 may play a functional role in choroidal and hippocampal response to brain injury. It is also suggested that GPR125 may be a marker for spermatogonial stem cells. Four isoforms of GPR125 exists due to alternative splicing events. |
| Swiss Prot | Q8IWK6 |
| Gene ID | 166647 |
| Subcellular Location | Cell membrane; Multi pass membrane protein |
| Immunogen | A synthetic peptide of human GPR125 |
| Public Immunogen Range | 422-530/1321aa |
| Calculated MW | 143kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse |
| Application | ELISA FCM IF ICC IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with GPR125 antibody diluted at 1:1000;Lane: Mouse Spinal cord.
Immunohistochemistry of paraffin-embedded mouse brain tissue with PNPT1 antibody diluted at 1:400
Immunohistochemistry of paraffin-embedded mouse brain tissue with GPR125 antibody diluted at 1:400.
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