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My CartReactivity：Human,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：1576
Host：Rabbit
Qty：
Size：
| Name | Anti-CYP3A4 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. ;IHC 1:100-300. |
| Target | CYP3A4 |
| Background | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide. |
| Swiss Prot | Q6GRK0 |
| Gene ID | 1576 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human CYP3A4 |
| Public Immunogen Range | 250-504aa |
| Calculated MW | 57kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CYP3A4 antibody diluted at 1:2000;Lane: SHSY5Y,Rat liver.
Immunohistochemistry of paraffin-embedded Human liver cancer with CYP3A4 antibody diluted at 1:200.
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