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My CartReactivity：Mouse,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：80005
Host：Rabbit
Qty：
Size：
| Name | Anti-DOCK5 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000;IHC 1:100-300. |
| Target | DOCK5 |
| Background | Guanine nucleotide exchange factor (GEF) for Rho and Rac. GEF proteins activate small GTPases by exchanging bound GDP for free GTP (By similarity). Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed:19004829). |
| Swiss Prot | Q9H7D0 |
| Gene ID | 80005 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | Recombinant protein of human DOCK5 |
| Public Immunogen Range | 1-351aa |
| Calculated MW | 40/215kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Mouse Human |
| Application | IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with DOCK5 antibody diluted at 1:2000;Lane: A549.
Immunohistochemistry of paraffin-embedded Human breast cancer with DOCK5 antibody diluted at 1:200.
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