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My CartReactivity：Mouse,Rat,Human
Application：IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：6478
Host：Rabbit
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| Name | Anti-SIAH2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Zebrafish;Xenopus laevis |
| Dilution Ratio | IHC 1:50-200. |
| Target | SIAH2 |
| Background | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:9334332, PubMed:11483518, PubMed:19224863). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:9334332, PubMed:11483518, PubMed:19224863). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PubMed:9334332, PubMed:11483518, PubMed:19224863). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP) (PubMed:9334332, PubMed:11483518, PubMed:19224863). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia (PubMed:22878263). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes (PubMed:9334332, PubMed:11483518, PubMed:19224863, PubMed:22878263). Has some overlapping function with SIAH1 (PubMed:9334332, PubMed:11483518, PubMed:19224863). Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not (PubMed:12411493). Promotes monoubiquitination of SNCA (PubMed:19224863). Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation (PubMed:26392558). Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance (PubMed:26392558). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 (By similarity). |
| Swiss Prot | O43255 |
| Gene ID | 6478 |
| Subcellular Location | Nucleus Cytoplasm |
| Immunogen | A synthetic peptide of human SIAH2 |
| Public Immunogen Range | 105-155/324aa |
| Calculated MW | 35kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human stomach cancer with SIAH2 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human breast cancer with SIAH2 antibody diluted at 1:100.
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