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My CartReactivity：Human,Rat,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：51002
Host：Rabbit
Qty：
Size：
| Name | Anti-TPRKB Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:200-1000. IHC 1:100-300. |
| Target | TPRKB |
| Background | Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an allosteric effector that regulates the t6A activity of the complex. TPRKB is not required for tRNA modification. |
| Swiss Prot | Q9Y3C4 |
| Gene ID | 51002 |
| Subcellular Location | Cytoplasm Cell membrane Nucleus |
| Immunogen | Recombinant protein of human TPRKB |
| Public Immunogen Range | 1-175aa |
| Calculated MW | 16/20/24kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TPRKB antibody diluted at 1:500;Lane: HeLa,A549,MCF7.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with TPRKB antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded Human thyroid cancer with TPRKB antibody diluted at 1:200.
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