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My CartReactivity：Mouse,Human,Monkey
Application：WB;IHC;IF,WB,IHC,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：56478
Host：Rabbit
Qty：
Size：
| Name | Anti-EIF4ENIF1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. IF 1:50-200. |
| Target | EIF4ENIF1 |
| Background | EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:24335285, PubMed:32354837). Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis. Promotes miRNA-mediated translational repression. Required for the formation of P-bodies. |
| Swiss Prot | Q9NRA8 |
| Gene ID | 56478 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human EIF4ENIF1 |
| Public Immunogen Range | 1-350aa |
| Calculated MW | 88/108kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Monkey Mouse |
| Application | IF WB;IHC;IF WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with EIF4ENIF1 antibody diluted at 1:2000;Lane: U-251,MCF7,Hela,HepG2,THP-1,LO2,293T,COS-7.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with EIF4ENIF1 antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with EIF4ENIF1 antibody diluted at 1:200.
Immunofluorescence analysis of NIH3T3 cells using EIF4ENIF1 antibody diluted at 1:100.
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