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Ketone Body Content Assay Kit

BC5060-241212Manual

Cat.No：BC5060 Solarbio

Detection Method：Spectrophotometer

Storage：Store at -20℃，6 months

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Product Information

Name	Ketone Body Content Assay Kit
Detection Method	Spectrophotometer
Storage	Store at -20℃，6 months
Unit	Kit
Specification	50T/48S

Determination of Significance：

Ketone bodies are intermediate products of fatty acid oxidative decomposition in liver. It includes Acetoacetic acid (AcAc) and β- Hydroxybutyric acid (BOH) and acetone. The amount of acetone in ketone body is very small, and it is absorbed immediately. AcAc and BOH is oxidized in extrahepatic tissue through blood flow. The citric acid cycle provides more energy for those tissues, such as bone, myocardium and renal cortex.

Measurement Principle：

At pH 7.0 and 37℃, β- Hydroxybutyrate dehydrogenase (HBDH) catalyzes the dehydrogenation of BOH to produce phthalic acid, and NAD+ is reduced to NADH. At pH 8.8 and 37℃, HBDH reduced AcAc to 3-hydroxybutyrate or decarboxylated to acetone, and NADH was oxidized to NAD+. NADPH has a characteristic absorption peak at 340nm. The content of BOH and AcAc can be calculated by detecting the change of absorbance at 340nm. Then the content of ketone body in the sample can be calculated.

Self Provided：

Ultraviolet spectrophotometer, desk centrifuge, pipette, 1mL quartz cuvette, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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