{{cart_num}}
My CartReactivity：Human,Rat,Mouse
Clone Type：Monoclonal antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4301
Host：Mouse
Qty：
Size：
| Name | Anti-AF-6（B-5） Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal antibody |
| Subtype | IgG2aK |
| Clone Number | B-5 |
| Dilution Ratio | WB 1:100-1000. IP 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate). IF 1:50-500. IHC 1:50-500. ELISA 1:30-3000. |
| Target | AF-6 |
| Background | The dynamics of a cell-cell interface such as tight junctions or adherensjunctions are important in many developmental, physiological and pathological processes. AF-6 (MLLT4: myeloid/lymphoid or mixed-lineage leukemia translocated to 4) is a 1,612 amino acid protein that contains two N-terminal Ras binding domains (RBD) and a GLGF motif, and is implicated in Ras-mediated signaling events occurring at peripheral cell-cell junctions. AF-6 interacts with F-Actin and Profilin in cell-cell junctions, and may modulate Actin modeling near adhesion complexes. Furthermore, AF-6 coordinates junction adhesion molecule (JAM) recruitment to intercellular junctions through a PDZ domain. Developing mice deficient in AF-6 activity display a loss of neuroepithelial polarity, suggesting that AF-6 activity is an important regulator of cell-cell junctions that influence apical/basolateral asymmetry |
| Swiss Prot | P55196 |
| Gene ID | 4301 |
| Subcellular Location | Cell junction, adherens junction |
| Immunogen | Recombinant fusion protein of Human AF-6 |
| Calculated MW | 207kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:100-1000. IP 1:20. IF 1:50-500. IHC 1:50-500. ELISA 1:30-3000. |
| Specification | 1ml |
| Reactivity | Human Rat Mouse |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with AF-6(B-5) antibody diluted at 1:1000;Lane:HepG2
Immunohistochemistry of paraffin-embedded Human testis with AF-6(B-5) antibody diluted at 1:200
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
