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My CartReactivity：Rat,Mouse,Human
Application：ELISA,FCM,IF,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1027
Host：Rabbit
Qty：
Size：
| Name | Anti-CDKN1B/p27 KIP1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chicken;Dog;Pig;Sheep |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IP 1:20-100. IHC 1:100-500. FCM 1μg/Test . IF 1:200-800. |
| Target | CDKN1B/p27 KIP1 |
| Background | Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. |
| Swiss Prot | P46527 |
| Gene ID | 1027 |
| Subcellular Location | Nucleus Cytoplasm |
| Immunogen | A synthetic peptide of human CDKN1B/p27 KIP1 |
| Public Immunogen Range | 101-198/198aa |
| Calculated MW | 22kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CDKN1B/p27 KIP 1 antibody diluted at 1:1000;Lane: 293T.
Immunofluorescence analysis of MCF7 cell using CDKN1B antibody diluted at 1:200.
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