{{cart_num}}
My Cart> Antibodies > Primary Antibodies > Anti-Phospho-RELA(Ser276) Polyclonal AntibodyReactivity：Rat,Mouse,Human
Application：ICC,IHC,ELISA,FCM,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5970
Host：Rabbit
Qty：
Size：
| Name | Anti-Phospho-RELA(Ser276) Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Dog;Pig;Cow;Horse |
| Dilution Ratio | ELISA 1:5000-10000. IHC 1:100-500. FCM 0.2μg /Test. ICC 1:100. IF 1:100-500. |
| Target | Phospho-RELA(Ser276) |
| Background | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. |
| Swiss Prot | Q04206 |
| Gene ID | 5970 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human RELA around the phosphorylation site of Ser276 |
| Calculated MW | 59/60kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF ICC IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Rat urinary bladder with phospho-RELA(Ser276) antibody diluted at 1:400
Immunohistochemistry of paraffin-embedded Rat uterus with phospho-RELA(Ser276) antibody diluted at 1:200
Immunofluorescence analysis of MCF7 cells using phospho-RELA(Ser276) antibody
Immunohistochemistry of paraffin-embedded Rat urinary bladder with Phospho-NFKB p65(Ser276) antibody diluted at 1:400.
Immunofluorescence analysis of MCF7 cell using Phospho-NFKB p65 (Ser276) antibody diluted at 1:100.
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
