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My Cart> Antibodies > Primary Antibodies > Anti-Phospho-IRAK1(Thr387) Polyclonal AntibodyReactivity：Rat,Mouse,Human
Application：IHC,WB,ELISA,FCM,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3654
Host：Rabbit
Qty：
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| Name | Anti-Phospho-IRAK1(Thr387) Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Dog;Pig;Cow;Rabbit |
| Dilution Ratio | WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 0.2μg /Test. IF 1:100-500. |
| Target | Phospho-IRAK1(Thr387) |
| Background | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. |
| Swiss Prot | P51617 |
| Gene ID | 3654 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human IRAK1 around the phosphorylation site of Thr387 |
| Calculated MW | 77kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with Phospho-IRAK1 (Thr387) antibody diluted at 1:200;Lane: Mouse Liver Human Colon carcinoma.
Immunohistochemistry of paraffin-embedded Mouse spleen with Phospho-IRAK1(Thr387) antibody diluted at 1:200
Immunohistochemistry of paraffin-embedded Mouse brain with Phospho-IRAK1 (Thr387) antibody diluted at 1:400.
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