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My CartReactivity：Rat,Human,Mouse
Application：WB,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2852
Host：Rabbit
Qty：
Size：
| Name | Anti-GPER1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. |
| Target | GPER1 |
| Background | This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. |
| Swiss Prot | Q99527 |
| Gene ID | 2852 |
| Subcellular Location | Basolateral cell membrane Cell junction Cell membrane Cell projection Cytoplasm Cytoplasmic vesicle membrane Early endosome Endoplasmic reticulum membrane Golgi apparatus Golgi apparatus membrane Mitochondrion membrane Multi-pass membrane protein Nucleus Recycling endosome axon cytoskeleton dendrite dendritic spine membrane perinuclear region postsynaptic cell membrane postsynaptic density synapse trans-Golgi network |
| Immunogen | Recombinant protein of human GPER1 |
| Public Immunogen Range | 307-375aa |
| Calculated MW | 42kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.03% Proclin300，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human Mouse |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human liver cancer with GPER1 antibody diluted at 1:100
Western blot analysis with GPER1 antibody diluted at 1:1000;Lane: Mouse heart.
Immunofluorescence analysis of NIH3T3 cells using GPER1 antibody diluted at 1:100.
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