Tuner(DE3)Competent Cell
Cat.No：C1150 Solarbio
Storage：Store at -70℃
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Molecular Biology > Recombinant Clone Expression > Competent Cell > Tuner(DE3)Competent Cell
Product Information
| Name | Tuner(DE3)Competent Cell |
| Storage | Store at -70℃ |
| Unit | Piece |
| Specification | 10×100μl |
Tuner(DE3) was a LacYZ mutant strain BL21(DE3). Tuner(DE3) strains can accurately control the expression of recombinant protein by the addition of IPTG. When combined with pET series carriers, the protein expression can be effectively regulated from low expression level to high expression level (when low concentration IPTG is used to induce low expression of recombinant protein, The target protein may have better solubility and biological activity). This strain contains the DE3 region of λ phage, which can express T7 RNA polymerase. Tuner(DE3) strains belong to B strains, lon and ompT protease deficient types. Tuner(DE3) receptive cells were prepared by a special process, and the conversion efficiency of pUC19 plasmid was 107cfu/μg DNA.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Publish_to：CHEMISTRY & BIODIVERSITY
PMID：37294554
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