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My Cart> Antibodies > Primary Antibodies > Anti-Phospho-Smad2-S465/467 + Smad3-S423/425 Polyclonal AntibodyReactivity：Rat,Mouse,Human
Application：WB,IHC,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20°C. Avoid freeze / thaw cycles.
Gene ID：4087/4088
Host：Rabbit
Qty：
Size：
| Name | Anti-Phospho-Smad2-S465/467 + Smad3-S423/425 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000.IHC 1:50-200.IF 1:50-200. |
| Target | Smad2-S465/467 + Smad3-S423/425 |
| Background | SMAD proteins mediate the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. |
| Swiss Prot | Q15796/P84022 |
| Gene ID | 4087/4088 |
| Subcellular Location | Cytoplasm,Nucleus |
| Immunogen | A synthetic phosphorylated peptide around S465 & S467 of human Smad2 |
| Calculated MW | 48/52/25/35/43kDa |
| Purification Method | Affinity purification |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage | Store at -20°C. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with Smad2-S465/467 + Smad3-S423/425 antibody diluted at 1:1000;Lane: C2C12.
Immunohistochemistry of paraffin-embedded human colon carcinoma with Smad2-S465/467 + Smad3-S423/425 antibody diluted at 1:100.
Immunofluorescence analysis of L929 cells using Smad2-S465/467 + Smad3-S423/425 antibody diluted at 1:100.
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