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My CartReactivity：Human,Rat,Mouse
Application：WB,IHC,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：4702
Host：Rabbit
Qty：
Size：
| Name | Anti-NDUFA8 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:5000-8000. IHC 1:50-200. IF 1:50-200. |
| Target | NDUFA8 |
| Background | The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. |
| Swiss Prot | P51970 |
| Gene ID | 4702 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human NDUFA8 |
| Public Immunogen Range | 120-170/172aa |
| Calculated MW | 20kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with NDUFA8 antibody diluted at 1:6000;Lane: HepG2,293T,LO2,Mouse heart,Rat brain.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with NDUFA8 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human liver cancer with NDUFA8 antibody diluted at 1:100.
Immunofluorescence analysis of SH-SY5Y cells using NDUFA8 antibody diluted at 1:100.
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