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My Cart> Antibodies > Loading Control Antibodies > Nucleus > Anti-PCNA Monoclonal AntibodyReactivity：Human,Rat,Mouse
Application：IHC;WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：5111
Host：Mouse
Qty：
Size：
| Name | Anti-PCNA Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 12D10 |
| Dilution Ratio | WB 1:2000-5000. IHC 1:50-300. |
| Target | PCNA |
| Background | PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| Swiss Prot | P12004 |
| Gene ID | 5111 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide coupled to KLH |
| Calculated MW | 29kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IHC;WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PCNA antibody diluted at 1:3000;Lane: HeLa,293T,Jurkat,HepG2,A549,MCF7,NIH3T3,Rat testis.
Immunohistochemistry of paraffin-embedded Human cervical cancer with PCNA antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded Human ovarian cancer with PCNA antibody diluted at 1:200.
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MicroRNA?153 attenuates hypoxia?induced excessive proliferation and migration of pulmonary arterial smooth muscle cells by targeting ROCK1 and NFATc3
Click to check >>Author：Zhao M
IF：2.9520
Publish_to：Molecular medicine reports
PMID：33495839
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