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My CartReactivity：Mouse,Rat,Human
Application：WB,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：9184
Host：Rabbit
Qty：
Size：
| Name | Anti-BUB3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:50-200. IF 1:50-200. |
| Target | BUB3 |
| Background | This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Swiss Prot | O43684 |
| Gene ID | 9184 |
| Subcellular Location | Chromosome Nucleus centromere kinetochore |
| Immunogen | Recombinant protein of human BUB3 |
| Public Immunogen Range | 1-328aa |
| Calculated MW | 37kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | "WB 1:500-2000. IF 1:50-200." |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunofluorescence analysis of U20S cells using BUB3 antibody diluted at 1:100.
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