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My CartReactivity：Mouse,Rat,Human
Application：ICC,IP,IF,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3309
Host：Mouse
Qty：
Size：
| Name | Anti-HSPA5 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2b |
| Clone Number | 4F11-D5-C10 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Target | HSPA5 |
| Background | The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. |
| Swiss Prot | P11021 |
| Gene ID | 3309 |
| Subcellular Location | Cytoplasm Endoplasmic reticulum lumen Melanosome |
| Immunogen | Recombinant protein of human HSPA5 |
| Calculated MW | 78kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | ICC IP IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HSPA5 antibody diluted at 1:1000;Lane: NIH3T3,A549,C6,HeLa.
Immunofluorescence analysis of HeLa cells using HSPA5 antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using HSPA5 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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