RNase A
Cat.No：R8020 Solarbio
CAS：9001-99-4
Appearance：White freeze-dried powder
Storage：Store at -20℃，3 years
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
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MAPK
NF-κB
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TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | RNase A |
| CAS | 9001-99-4 |
| Storage | Store at -20℃，3 years |
| Appearance | White freeze-dried powder |
| Source | Bovine pancreas |
| Enzyme Activity/Titer | Assay: approx. 70 % ,Activity: min. 70 U/mg (Kunitz) |
| Solubility | 10 mg/mL RNA dissolved liquid |
| Unit | Bottle |
| Specification | 25mg 100mg 1g |
Product introduction
Ribonuclease A is an endoribonuclease that specifically attacks the 3' end of pyrimidine residues on RNA and clears phosphodiester bonds formed with neighboring nucleotides. The end products of the reaction are pyrimidine 3' phosphate and an oligonucleotide with a terminal pyrimidine 3' phosphate. "In the absence of cofactors and divalent cations, rnase A action can be inhibited by placental ribonuclease inhibitors (B1ackburn et al. 1977) or oxovadium-ribonucleoside complexes (Puskas et al. 1982)."
Scope of application
The structure of nucleic acid was determined. RNA sequence analysis; Hydrolyze RNA in protein samples; Purified DNA.
Usage method
The RNase A powder was dissolved in 0.01M NaAc PH5.2 to a final concentration of 10mg/mL, heated in a boiling water bath at 100 ° C for 15min, and slowly cooled at room temperature. The PH was adjusted to about 7.4 with 0.1 volume of 1M Tris-HCl and stored at -20 ° C in aliquots. RNase A precipitates when the concentrated RNase A solution is heated to 100 ° C at neutral PH. (Note: from the Guide to Molecular Cloning Experiments, 3rd Ed, page 1647)
Matters needing attention
Biochemical research supplies
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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