MC3T3-E1 Subclone 14
Cat.No:SCC-220913 Solarbio
Storage:Store at -70℃
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| Name | MC3T3-E1 Subclone 14 |
| Full Name | Precranial bone cell subcloning in mice 14 |
| Storage | Store at -70℃ |
| Transport Condition | Dry ice transport |
| Culture Condition | MEMα+10% FBS+1% P/S |
| Cell Type | Adherent cell |
| Background | A series of subclones were isolated from cloned but phenotypically distinct MC3T3-E1 cell lines. High or low osteoblast differentiated and mineralized subclones were selected from osteoblasts grown with ascorbic acid media. MC3T3-E1 Subclone 4 and MC3T3-E1 Subclone 14 showed high levels of osteoblast differentiation in ascorbic acid and 3-4mM inorganic phosphate growth. They form a well mineralized extracellular matrix 10 days later MC3T3-E1 Subclone24 and MC3T3-E1 Subclone30 show poor osteoblast differentiation in ascorbic acid. ECM is not formed and can be used as a negative contrast between MC3T3-E1 Subclone 4 and MC3T3-E1 Subclone 14. Expression of mineralized subclonal selection as an osteoblast-labeled mRNA and sialic glycoprotein (BSP), osteocalcin (OCN) and mRNA of parathyroid hormone/parathyroid hormone-related protein receptors. High or low differentiation potential subclones produce similar amounts of collagen in culture, expressing comparable basic levels of mRNA coding Osf2/Cbfa1, an osteoblast-related transcription factor. After the implantation of immunodeficiency mice, highly differentiated subclones formed a small bone-forming braided bone similar to the bone, and low differentiated cells only produced fibrous tissue. These cell lines are a good model for studying osteoblast differentiation in vitro, especially ECM signals. They behave similarly to primary culture of cranial apical osteoblasts. |
| Source | Cranial crest |
| Unit | Piece |
| Specification | 1EA |
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
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