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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：15.625pg/mL
Storage：2-8℃，6 months
Gene ID：5657
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| Name | Human Proteinase 3/Myeloblastin/PRTN3 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Proteinase 3/Myeloblastin/PRTN3 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 31.25-2000pg/mL |
| Sensitivity | 15.625pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | P24158 |
| Gene ID | 5657 |
| Background | Proteinase 3 (PRTN3)，also known as Myeloblastin (MBN)，is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils，the latter of which either secretes it，sequesters it in azurophilic granules，or expresses it on the cell surface. When secreted，it acts on HK and activates the kinin pathway. In azurophilic granules，it aids in the digestion of phagocytosed material. On the cell surface，it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation，a 221 aa mature enzyme (aa 28-248)，and an eight aa C?terminal propeptide (aa 249-256). Within the cell，a 35 kDa immature form exists; on the cell surface，both constitutively inactive，and induced active forms may be found，often in a noncovalent association with CD177/NB1. Over aa 26-249，human PRTN3 shares 68% aa identity with mouse PRTN3. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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