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My CartReactivity：Mouse,Human,Rat
Application：IP,WB,IF,IHC,ELISA,FCM
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：100506658
Host：Rabbit
Qty：
Size：
| Name | Anti-Occludin Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-16000. IHC 1:50-800. IF 1:200-800. IP 0.5-4.0ug. FCM 1X10^6 cells+0.2ug. |
| Target | Occludin |
| Background | Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074). |
| Swiss Prot | Q16625 |
| Gene ID | 100506658 |
| Subcellular Location | Cell membrane |
| Immunogen | Recombinant protein of human Occludin |
| Public Immunogen Range | 269-522aa |
| Calculated MW | 59kDa |
| Observed MW | 59kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC IF IP FCM ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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