Recombinant RNaseA
Cat.No:R8022 Solarbio
Purity:≥ 90%
Storage:Store at -20℃,3 years
Appearance:Light yellow Lyophilization
CAS:9001-99-4
Molecular Weight:14.3kDa
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Purity:≥ 90%
Storage:Store at -20℃,3 years
Appearance:Light yellow Lyophilization
CAS:9001-99-4
Molecular Weight:14.3kDa
Qty:
Size:
| CAS | 9001-99-4 |
| Appearance | Light yellow Lyophilization |
| Purity | ≥ 90% |
| Storage | Store at -20℃,3 years |
| Molecular Weight | 14.3kDa |
| Unit | Piece |
| Specification | 10mg 50mg 200mg |
Introduction
RibonucleaseA (RNase A) is A non-specific endonuclease derived from bovine pancreas modified by protein engineering technology and obtained by yeast expression and purification. Functionally, RNase A is an endonuclease that hydrolyzes the phosphodiester bond between the 5 '-ribose on the nucleoside and the phosphate group on the adjacent 3' -ribose pyrimidine nucleoside, resulting in the hydrolysis of the 2 ',3 '-cyclic phosphoric acid to the corresponding 3' -nucleoside phosphate. Therefore, single-stranded RNA can be specifically degraded at the sites of C and U nucleotide residues. RNase A is very stable. RNase A is most efficient when it acts on single-stranded RNA.
Application
Remove RNA contamination from DNA or protein samples.
How to use
Storage concentration:10 mg/mL.
The working concentration:10-20μg/mL, the working liquid should be diluted with diluent or the working substrate should be 20 mM Mg2+.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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