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My Cart> Antibodies > Primary Antibodies > Anti-Phospho-PERK (Thr982) Polyclonal AntibodyReactivity：Rat,Human,Mouse
Application：ELISA,WB,IF,ICC,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：9451
Host：Rabbit
Qty：
Size：
| Name | Anti-Phospho-PERK (Thr982) Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. IF 1:50-200. ELISA 1:10000. |
| Target | Phospho-PERK (Thr982) |
| Background | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. |
| Swiss Prot | Q9NZJ5 |
| Gene ID | 9451 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human Phospho-PERK (Thr982) |
| Public Immunogen Range | 947-996aa |
| Calculated MW | 125kDa |
| Observed MW | 130kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% glycerol，0.5% BSA and 0.02% sodium azide，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human Mouse |
| Application | ELISA ICC IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with Phospho-PERK (Thr982) antibody diluted at 1:1000;Lane: A453,AD293,HeLa.
Immunohistochemistry of paraffin-embedded Mouse kidney with Phospho-PERK (Thr982) antibody diluted at 1:100.
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