Goat anti-Rabbit IgG
Cat.No:SPA134 Solarbio
Reactivity:Rabbit
Application:WB,ELISA
Clone Type:Polyclonal Antibody
Appearance:Liquid
Storage:Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
Host:Goat
Qty:
Size:
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My Cart> Antibodies > Secondary Antibodies > Purified Secondary Antibodies > Goat anti-Rabbit IgGReactivity:Rabbit
Application:WB,ELISA
Clone Type:Polyclonal Antibody
Appearance:Liquid
Storage:Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
Host:Goat
Qty:
Size:
| Name | Goat Anti-Rabbit IgG(Purified Antibody) |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-20000.;ELISA:1:5000-100000. |
| Background | Immunoglobulin G (IgG),is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Immunogen | Rabbit IgG full length protein |
| Calculated MW | 150kDa |
| Purification Method | Affinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300(PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
| Appearance | Liquid |
| Concentration | 10mg/mL |
| Unit | Piece |
| Y-Application | ELISA WB |
| Y-Dilution Ratio | WB 1:1000-20000.;ELISA:1:5000-100000. |
| Specification | 1mg 10mg 100mg |
| Reactivity | Rabbit |
| Application | WB ELISA |
Product introduction:
This product is purified by Protein A/G affinity chromatography to produce purified sheep or rabbit IgG antibodies with high purity (>95%) and high activity.
This product is a PBS solution, which can be prepared into different concentrations according to user requirements. It is best to store at -20℃ before use, and different concentrations can be diluted according to the use method.
Usually different purposes can be diluted with the following buffer solution, pay attention to fully dissolved after mixing. Frozen for a long time will produce a small amount of polymer, it is best to centrifuge at low temperature and high speed before use, remove cold polymerized globulin and denatured protein after quantification.
Antibody label: 500ug ~ 20mg/ml, ELISA plate: 10 ~ 20ug/ml, chromatographic gold bar control line: 2mg/ml, etc.
Colloidal gold label: Diluted with pH9.0 boric acid buffer.
HRP labeling: Diluted with 0.01mol/L pH9.0CB or coated solution CB.
Fluorescein labeling: 0.5mol/L Ph 9.5CB (carbonate buffer) dilution.
Biotin labeling: diluted with 0.1mol/L NaHCO3.
Other methods: such as immunodiffusion can be diluted with 0.01mol/L pH7.2PBS.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
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