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My CartReactivity：Human,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：124454
Host：Rabbit
Qty：
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| Name | Anti-EARS2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Zebrafish;Chicken;Monkey |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | EARS2 |
| Background | This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants. |
| Swiss Prot | Q5JPH6 |
| Gene ID | 124454 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human EARS2 |
| Public Immunogen Range | 1-350aa |
| Calculated MW | 59/60kDa |
| Observed MW | 58kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with EARS2 antibody diluted at 1:3000;Lane: K-562,T47d,HEK293,Jurkat.
Immunohistochemistry of paraffin-embedded Human testis cancer with EARS2 antibody diluted at 1:50.
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