Anti-ATG4A Polyclonal Antibody
Cat: K108283P
Summary:
| 【Product name】：Anti-ATG4A Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：115201 | 【Swiss Prot】：Q8WYN0 |
| 【Tested applications】：WB,IHC | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:50-200. | |
| 【Immunogen】：A synthetic peptide of Human ATG4A | |
| 【Public Immunogen Range】：70-120/398aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes; which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Verified picture:
Western blot analysis with ATG4A antibody diluted at 1:2000;Lane: Jurkat,K562,MCF7,MDA-MB-231.
Immunohistochemistry of paraffin-embedded Human breast cancer with ATG4A antibody diluted at 1:100.