Anti-VIM Monoclonal Antibody
Cat: K000165M
Summary:
| 【Product name】：Anti-VIM Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG2a | 【Purification】：Affinity purification |
| 【Gene ID】：7431 | 【Swiss Prot】：P08670 |
| 【Tested applications】：ICC,IP,IF,IHC,WB | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:50-100. IF 1:50-200. IP 1:20. ICC 1:50-200. | |
| 【Immunogen】：Recombinant protein of human VIM | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Verified picture:
Western blot analysis with VIM antibody diluted at 1:1000;Lane: MOLT-4,Raji,K562,Jurkat,HeLa.
Immunohistochemistry of paraffin-embedded Human tonsils with VIM antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using VIM antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using VIM antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.