Anti-EGFR Monoclonal Antibody
Cat: K000140M
Summary:
| 【Product name】：Anti-EGFR Monoclonal Antibody | 【Source】：Mouse |
| 【Isotype】：IgG1 | 【Purification】：Affinity purification |
| 【Gene ID】：1956 | 【Swiss Prot】：P00533 |
| 【Tested applications】：IF,WB,ICC,IP | |
| 【Recommended dilution】：WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200. | |
| 【Immunogen】：Recombinant protein of human EGFR | |
| 【Public Immunogen Range】：25-645aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Verified picture:
Western blot analysis with EGFR antibody diluted at 1:1000;Lane: COS7,A549.
Immunofluorescence analysis of HeLa cells using EGFR antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using EGFR antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.