Anti-PC Polyclonal Antibody
Cat: K112732P
Summary:
| 【Product name】：Anti-PC Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：5091 | 【Swiss Prot】：P11498 |
| 【Observed】：125kDa | |
| 【Tested applications】：IHC,WB | |
| 【Recommended dilution】：WB 1:1000-3000. IHC 1:100-300. | |
| 【Immunogen】：A synthetic peptide of Human PC | |
| 【Public Immunogen Range】：790-840/1178aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
Verified picture:
Western blot analysis with PC antibody diluted at 1:2000;Lane: PC-12,HepG2,MCF7,HeLa,A549,SH-SY5Y,K-562,Mouse liver,Mouse brain,Rat brain.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with PC antibody diluted at 1:200.