Anti-NLRP3 Polyclonal Antibody
Cat: K010074P
Summary:
| 【Product name】：Anti-NLRP3 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：114548 | 【Swiss Prot】：Q96P20 |
| 【Observed】：110kDa | |
| 【Tested applications】：IHC,WB,IF | |
| 【Recommended dilution】：WB 1:500-1000. IHC 1:200-400. IF 1:200-400. | |
| 【Immunogen】：A synthetic peptide of human NLRP3 | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
NALP3 is a component of the innate immune system that functions as a pathogen recognition receptor (PRR) that recognizes pathogen-associated molecular patterns (PAMPs). NALP3 together with the adaptor ASC protein PYCARD forms a caspase-1 activating complex known as the NALP3 inflammasome. NALP3 in the absence of activating signal is kept in an inactive state complexed with HSP90 and SGT1 in the cytoplasm. NALP3 inflammasome detects danger signals such as crystallineuric acid and extracellular ATP released by damaged cells. These signals release of HSP90 and SGT1 from and recruit ASC protein and caspase-1 to the inflammasome complex.
Verified picture:
Western blot analysis with NLRP3 antibody diluted at 1:500;Lane: THP-1 RAW264.7 Mouse spleen+LPS Mouse thymus Rat spleen+LPS.
Immunohistochemistry of paraffin-embedded mouse spleen with NLRP3 antibody diluted at 1:300.