Anti-Ki67 Polyclonal Antibody
Cat: K010071P
Summary:
| 【Product name】：Anti-Ki67 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：4288 | 【Swiss Prot】：P46013 |
| 【Tested applications】：IHC,IF | |
| 【Recommended dilution】：IHC 1:400-1000. IF 1:400-1000. | |
| 【Immunogen】：A synthetic peptide of human Ki67 | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).
Verified picture:
Immunohistochemistry of paraffin-embedded human colon cancer with Ki67 antibody diluted at 1:500.
Immunofluorescence analysis of human intestinal cancer using Ki67 antibody diluted at 1:800.